Measuring molecular interactions in real time using surface plasmon resonance (SPR) technology
July 29-31, 2013
Foran Hall, George H. Cook Campus
- July 29: Introduction to Biacore Principles
- July 30-31: Hands-on instrument training and execution of a model experiment
The Introduction to Biacore Principles lecture will be taught by GE Healthcare on Monday, July 29. It is an all day lecture, open to the public and is free of charge. GE Healthcare will provide binders describing the Biacore principles for the attendees.
The instrument training session will be taught by Dr. Xiao-Ping Li in Dr. Nilgun Tumer's lab on Tuesday, July 30 and Wednesday, July 31.
You will use a Biacore chip to capture a protein and study its interaction with purified ribosomes using a Biacore 3000 and Biacore T200. The hands on training session on days 2 and 3 is subject to a fee of $500 per person to cover the cost of the chips and reagents and open to only six people.
A Core Facility has been established in Foran Hall on the George H. Cook Campus that will enable our students and faculty and outside users to have access to instrumentation whose purchase and maintenance costs would be cost prohibitive for any one investigator.
The instrumentation offered at the Core Facility is grouped according to applications and include Bio-imaging, Bio-sensing, Flow cytometry, Real-time PCR, and High throughput screening.
- Within the Bio-imaging application we offer a recently acquired Zeiss LSM 710 confocal microscope with seven lasers, and environmental chamber with CO2 and temperature control with a range from 4°C to 45°C. The Zeiss LSM 710 is housed in a separate microscopy suite. In addition epifluorescent microscopy using Olympus microscopes capable of imaging a wide variety of dyes is available. We are excited to announce the new addition of Typhoon FLA 9500 Biomolecular Imager for detection of fluorescence and radioactive labels and 2-D DIGE imaging for differential expression studies.
- Our Bio-molecular interaction analysis instrumentation includes two Biacore instruments, Biacore 3000 and T200, which utilize surface plasmon resonance technology allowing for label-free detection of macromolecular interactions and isothermal titration calorimetry (ITC) instrumentation, which includes an iTC200 for direct and label free measurement of binding affinity and thermodynamics.
- Flow cytometry is made easy with the recent purchase of the user-friendly Accuri C6 Flow Cytometer that uses two lasers, two scatter and four fluorescence detectors.
- Our real-time PCR instrumentation, StepOnePlus, comes from Applied Biosystems, the leader in real-time PCR. Our experienced staff can provide support in experimental design.
- High throughput screening applications are made possible with a Singer RoToR pinning robot that can dispense to and from solid or liquid media in 96- and 384-well formats with a throughput of up to 100 plates per hour and a BioTek Synergy™ 4 microplate reader for fluorescence Intensity, fluorescence polarization, time-resolved fluorescence, luminescence and uv-visible absorbance measurements.
- Short courses on Biacore applications and real-time PCR are offered. These are hands-on courses where participants receive personal training on the instruments. Please contact us for more information.
This facility offers exceptional opportunities for research and training, and provides our students and staff with leading edge technology and state-of-the-art instrumentation for their research needs.
School of Environmental and Biological Sciences
George H. Cook Campus
Rutgers, The State University of New Jersey
New Brunswick, NJ 08901-8525